Nona Semana Integrada do Instituto de Física de São Carlos

Transport phenomena of a single molecule through a nanopore

Não agendado

20m

Doutorado

Palestrante

Sr. Leandro Augusto Zago

Descrição

With advances in nanotechnology techniques, the necessary refinement has been achieved so that new devices emerge and allow the observation of phenomena involving a single molecule. In this way, the polymethyl methacrylate polymer (PMMA) will be used as the material (1) for the production of thin films (in the order of 100nm) by spin-casting technique, and nanometrc pores will be produced in a controlled manner, (FIB) which consists of accelerating heavy ions to the energies of a few tens of KeV and focusing them on a target. It will also be used an electrolytic cell, where measurements of transport phenomena (2) will be made, such as the diffusion of a single protein through the membrane, where characteristics such as diffusion velocity, length of the molecule and its conformation will be observed, using the flow variation of current as the molecule travels through the nanopore. For an in-depth study the problem will be modeled computationally, in this way we can observe theoretical results and confront them with those obtained experimentally, as well as having the freedom to vary aspects of the system as pore shape and morphological properties of study molecules. These new devices capable of observing the transitions of the isolated molecules (3) through the nanopore will be functionalized, so that a current-rectifying response can be obtained through asymmetric pores using the deposition of surface charges, aiming at the creation of ultra-sensitive sensing devices for certain proteins for the detection of diseases in the early stages quickly and inexpensively.

Referências

1 MOAZED, B; HASHEMI, M; ACHENBACH, S. Novel PMMA polymer-based nanopores capable of detection and discrimination between structurally different biomolecules. IEE Sensors Journal, v. 14, n. 9, p. 3292-3309, 2014.
2 PLETT, T. S. et al. Solid-state ionic diodes demonstrated in conical nanopores. Journal of Physical Chemistry C, v. 121, n. 11, p. 6170-6176, 2017.
3 TAN, S. et al. Detection of a single enzyme molecule based on a solid-state nanopore sensor. Nanotechnology, v. 27, n. 5, p. 155502-1-155502-11, 2016.