Palestrante
Descrição
The development of methodologies for early diagnosis of colorectal cancer (CCR) is relevant, since this type of cancer is the third in number of deaths in the world. (1) In this study, we developed an electrochemical biosensor to detect the bacterium Fusobacterium nucleatum (F. nucleatum), a microorganism whose strong relationship with CCR was recently discovered. (2) Specific DNA sequences for the recognition of F. nucleatum were immobilized in magnetic microparticles (dynabeads) for sample preconcentration, and employed in the detection. Disposable microfluidic devices based on carbon printed electrodes were used for the specific recognition of biomarkers with electrochemical techniques. Among the advantages of this electrode system is the use of small sample volumes, possibility of miniaturization and rapid analysis, which can generate a low-cost system for the early detection of CCR. To prove the efficiency of the detection, differential pulse voltammetry (DPV) technique was applied to provide a comparison between the changes that occur in the surface of the dynabeads during the process of construction and detection. The proposed devices were capable of detecting and differenciate the presence and the absence of complementary DNA down to 1 µM in buffer solution with good correlation (RSD = 7%).
Referências
1 LIM, J. M. et al. Electrochemical peptide sensor for diagnosing adenoma-carcinoma transition in colon cancer. Biosensors and Bioelectronics, v. 98, p. 330-337, Dec. 2017. doi: 10.1016/j.bios.2017.07.013.
2 BULLMAN, S. et al. Analysis of Fusobacterium persistence and antibiotic response in colorectal cancer. Science, v. 358, n. 6369, p. 1443-1448, Dec. 2017.
| Subárea | Física da Matéria Condensada |
|---|---|
| Apresentação do trabalho acadêmico para o público geral | Não |
