Palestrantes
Descrição
Infectious disease is a continuous treatment challenge for the hospitals. Ventilator Associated Pneumonia (VAP) is one of the most dangerous respiratory disease, because can lead the patient to death. The majority of the antibiotics can not destroy the cells from the developed biofilm at a surface of the endotracheal tube. The endotracheal tube is a tube which auxiliated the patient to breath.(1) The biofilm is a way of the bacteria to grow, formed by the cells and a matrix called extrapollimeric matrix that gives a protection to the cells against antibiotic agents, acid and temperature.(2) The photodynamic therapy is a solution for kill a biofilm of S. aureus cells using blue light, curcumin as a photosensitizer and cellular oxygen. Different formulations and different ways to delivered the photosensitizer were tested. A curcumin film, curcumin dissolved in the surfactant tween 80, a nanoskin soaked with curcumin and curcumin dissolved at dmso and alcohol were the formulation tested at S. aureus and Escherichia coli biofilm. The time of the biofilm developed (1-7 days of developed biofilm) as the concentration, incubation time of the curcumin and the dose light were tested to optimize the experiments. Different type of ilumination was tested, testing the irradiance and the time dose for kill the cells. An experimental designes were planned and the PDT was optimized.
Referências
1 PERKINS, S. D.; WOELTJE, K. F.; ANGENENT, L. T. Endotracheal tube biofilm inoculation of oral flora and subsequent colonization of opportunistic pathogens. International Journal Medical Microbiology, v. 300, n.7,p. 503-511,2010.
2 LINDSAY, D.; HOLY, A. Bacterial biofilms within the clinical setting: what healthcare professionals should know. Journal of Hospital Infection, v.64, n.4, p. 313-325,2006.
| Subárea | Óptica e Lasers |
|---|---|
| Apresentação do trabalho acadêmico para o público geral | Sim |
