Palestrante
Descrição
Photobiomodulation (PBM), a technique that is being used for over 40 years, is based on applying light in order to modify cellular processes such as proliferation, tissue regeneration and analgesia. These effects are strong evidences that PBM can modulate the cell cycle and increase tissue oxygenation, which are determinant factors in a radiotherapy result (Balcer-Kubiczek, E. K. 2012). Therefore, in this project, the investigation of the combination of these techniques to the development of a protocol that enhances tumor damage and treatment efficacy is proposed. In vitro experiments will enable the study of PBM in cell proliferation and cell cycle and its combination with radiotherapy, using the 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. From these experiments the protocols for in vitro analysis will be determined. An oral cavity cancer model will be applied, using the human squamous cell carcinoma cells SCC-25, and in vivo tumor induction will be performed in hairless mice. The aim of these experiments is the evaluation of the techniques combination in a model of higher biological complexity and the analysis of tumor damage and animal survival in order to propose a protocol that enhances the cancer response to ionizing radiation.
Referências
Balcer-Kubiczek, E. K. 2012. “Apoptosis in Radiation Therapy: A Double-Edged Sword.” Experimental Oncology 34 (3): 277–85.
| Subárea | Óptica e Lasers |
|---|---|
| Apresentação do trabalho acadêmico para o público geral | Não |
